Berlin 2018 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 64: Nanostructures, Nanostructuring and Nanosized Soft Matter II
CPP 64.10: Vortrag
Donnerstag, 15. März 2018, 12:00–12:15, PC 203
AC electrokinetic manipulation of nanoparticles and molecules — Eva-Maria Laux1, Xenia Knigge1, Christian Wenger2, Frank F. Bier1, and •Ralph Hölzel1 — 1Fraunhofer Institute for Cell Therapy and Immunology, Bioanalysis and Bioprocesses, Potsdam-Golm (IZI-BB), Germany — 2IHP GmbH - Leibniz Institute for Innovative Microelectronics, Frankfurt (Oder), Germany
AC electric fields are increasingly exploited for the separation of nanoparticles and molecules in micro- and nanoelectrode systems. AC electrokinetic phenomena like dielectrophoresis (DEP) and AC electroosmosis allow a robust non-contact spatial manipulation. Here we present results of dielectrophoretic experiments on nanoparticles and proteins in various electrode systems. Numerous protein species have been successfully manipulated: intrinsically fluorescing proteins like eGFP, DsRed and RPE, and fluorescently labeled BSA; specific antibody binding as well as enzymatic function are shown to be retained after DEP immobilisation. Depending on electrode size, molecules and nano-particles are isolated as singles between planar triangular electrodes or on arrays comprising up to 500,000 conical electrodes. Using fluorescence polarisation microscopy, permanent dielectrophoretic immobilisation of eGFP molecules is demonstrated with parallel alignment of the proteins' main axis along the electric field, and the relative orientation of the molecule's fluorophor is determined.