Berlin 2018 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 76: Focus: Polymers in Multi-Component and Aqueous Solutions II - organized by Jens-Uwe Sommer and Debashish Mukherji
CPP 76.6: Topical Talk
Freitag, 16. März 2018, 11:00–11:30, C 130
Diffusion of proteins in bicontinuous microemulsions: controlled soft nano-confinement — •Thomas Hellweg, Oliver Wrede, and Ralph Neubauer — Physikalische und Biophysikalische Chemie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany
The interior of cells is crowded with different objects and the diffusive behaviour of proteins often does not follow the normal Fick type diffusion, where the mean square displacement grows linearly in time <x2> ∝ t. The diffusion is considered to be sub-diffusive if <x2> ∝ tα (with α < 1) [1]. However, due to the complexity of the cellular matrix it is very difficult to control the crowding conditions or the confinement and to make systematic studies inside living cells.
Hence, to better understand the dependence of protein diffusion on a confining environment, we study the movement of a fluorescent protein (GFP+) through bicontinuous microemulsions via FCS. The sponge like microemulsion structure, which is characterized via small angle scattering, not only slows down the translational movement of the tracer particle with decreasing domain size but also changes the characteristics of the diffusion from "Fick like" to "anomalous"[1].
Additional relevance for such works arises due to the use of microemulsions as reaction media for enzymatically catalyzed reactions [2].
[1]R. Neubauer, S. Höhn, M. Dulle, A. Lapp, C. Schulreich, and T. Hellweg, Soft Matter 13 (2017), 1998
[2]S. Wellert et al., Euro. Biophysics J., 40 (2011) 761