Berlin 2018 – wissenschaftliches Programm
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KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur
KFM 27: Postersession KFM
KFM 27.23: Poster
Donnerstag, 15. März 2018, 15:00–17:00, Poster E
Harmonic nanoparticles as markers for in-vivo imaging — •Dustin Dzikonski1, Christian Kijatkin1, Kay Lammers2, Achim Paululat2, and Mirco Imlau1 — 1Department of Physics, Osnabrück University, Germany — 2Department of Zoology & Developmental Biology, Osnabrück University, Germany
Nanoscaled optical markers are versatile contrast agents in biological environments; especially fluorescing dyes have risen to a microscopy standard. However, rather rigid excitation-emission wavelengths as well as Stokes shifts towards the red spectrum pose severe limitations to this class of materials. Moreover, time-critical analysis is hindered due to photobleaching, blinking, and potentially long fluorescent lifetimes.
A solution is the use of harmonic, alkali niobate-based nanoparticles that offer unprecedented flexibility due to their photostability, instantaneous response, and frequency tunability in a vast range [C. Kijatkin et al., Photonics 2017, 4(1), 11]. In light of novel applications, we plan to utilize infrared radiation from femtosecond pulsed lasers in combination with harmonic generation to i) increase excitation depth due to the lower absorption in this range, ii) create visible up to UV light for detection and manipulation, and iii) allow for time-critical evaluation of dynamic systems. As a proof of principle, we present our studies on using lithium niobate nanoparticles as tracers via second harmonic generation of infrared radiation to visualize and quantify the hemolymph velocity flux inside the heart chamber of living Drosophila melanogaster as a model system. Financial support (DFG INST 190/165-1 FUGG) is gratefully acknowledged.