Erlangen 2018 – wissenschaftliches Programm
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Q: Fachverband Quantenoptik und Photonik
Q 62: Poster: Quantum Optics and Photonics V
Q 62.79: Poster
Donnerstag, 8. März 2018, 16:15–18:15, Redoutensaal
Capillary Electrophoresis of Single Proteins via Interferometric Scattering Microscopy — •Mahyar Dahmardeh1,2, Matthew P. McDonald1,2, and Vahid Sandoghdar1,2 — 1Max Planck Institute for the Science of Light, Staudtstraße 2, D-91058 Erlangen, Germany — 2Friedrich Alexander University Erlangen-Nuremberg, D-91058 Erlangen, Germany
Electrophoresis has been used as the gold standard assay for studying protein structures and proteomics. However, generally there are certain limitations inherent in the design such as the requirement for a large sample size, long experimental integration times and tedious post processing procedures. All of these make conventional electrophoresis techniques arduous and in some cases impossible to implement. Over the course of the last few years we have developed a label-free optical technique that senses individual nanoparticles and proteins using the interferometric detection of scattered light (iSCAT). iSCAT signals arise from interference between scattered waves and the light backreflected from the microscope cover-glass surface. Since iSCAT operates by way of single protein optical detection, combining it with electrophoresis overcomes the afore mentioned constraints. We have thus developed an electrophoresis based technique compatible with iSCAT-based detection. Initial measurements indicated that this approach is viable and single proteins have been synchronously detected in a prototype iSCAT based apparatus. This work will seek to establish a novel method as a robust, easy-to-use procedure that can markedly supplement existing electrophoretic techniques.