Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster II
BP 12.11: Poster
Dienstag, 2. April 2019, 14:00–16:00, Poster B2
Imaging of folded proteins deposited via soft-landing native electrospray ion beam deposition — •Sven Szilagyi1, Hannah Ochner1, Lukas Krumbein1, Joseph Gault2, Albert Konijnenberg3, Esther Martin3,4,5, Justin Benesch2, Frank Sobott3,4,5, Carol Robinson2, Sabine Abb1, Stephan Rauschenbach1,2, and Klaus Kern1,6 — 1Max-Planck-Institut für Festkörperforschung, Stuttgart — 2Department of Chemistry, University of Oxford — 3Department of Chemistry, University of Antwerp — 4Astbury Centre, University of Leeds — 5School of Molecular and Cellular Biology, University of Leeds — 6École Polytechnique Fédérale de Lausanne
Imaging techniques provide valuable information on the structure of proteins. Gaining reliable data strongly depends on the sample preparation technique, such as shock-freezing and staining of proteins or drop casting of samples. Here, we present the soft landing electrospray ion beam deposition (ES-IBD) as an alternative sample preparation approach in (ultra) high vacuum for imaging of protein samples. To this end, native electrospray ionization brings the proteins into the gas phase while preserving their folded state [1]. In this poster, we present successfully deposited proteins of different size which were imaged using different techniques, such as TEM, AFM, STM and Low Energy Electron Holography (LEEH) [2]. We further discuss the substrate influence on the folded state of the proteins from metal surfaces to free-standing graphene. [1] Nat. Meth., 5(11), 2008, 927-933. [2] PNAS, 114(7), 2017, 1474-1479.