Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster II
BP 12.12: Poster
Dienstag, 2. April 2019, 14:00–16:00, Poster B2
Force microscopy studies of mechanically loaded albumin films — •Lukas Böttcher1, Sven Kraft1, Regina Lange1, Ingo Barke1, Jessica Hembus2, Carmen Zietz2, Rainer Bader2, and Sylvia Speller1 — 1Institute of Physics, University of Rostock, 18051 Rostock — 2Biomechanics and Implant Technology Research Laboratory, University Medical Center Rostock, 18057 Rostock
In humans articulating joints such as hip or knee contain synovial fluid for sufficient lubrication. Major components are polysaccharides, lipids and proteins such as albumin. During walking oscillating pressure is applied leading to structural changes of the proteins. Under dynamic pressure the proteins might be partially unfolded or even formation of amyloid may occur. Such degraded protein possibly contributes to lubrication. Typical tip-sample pressures in atomic force microscopy are in the same regime as macroscopic joints. Our research question is whether a parameter can be derived from force spectra and be established as a measure for protein degradation. In an initial stage we use albumin as a model protein. This approach requires substantial energy transfer from an oscillating cantilever tip to the protein. In this work we present first results of topographic and spectroscopic analysis of mechanical-loaded albumin films on surfaces.