Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Poster II

BP 12.59: Poster

Dienstag, 2. April 2019, 14:00–16:00, Poster B2

Interaction of superparamagnetic iron oxide nanoparticles and transferrin — •Ulrike Martens¹, Ali Abou-Hassan², and Mihaela Delcea¹ — ¹Institute for Biochemistry/ZIK HIKE, Greifswald University, Germany — ²Laboratoire PHENIX, Sorbonne Université, Paris, France

Recent research demonstrated that nanoparticles (NPs) can enhance or suppress the immune response by binding to proteins of the blood stream. One important blood protein is transferrin which is composed of two distinct domains each containing an iron binding site. Its main role is to deliver iron to all biological tissues. By applying various biophysical techniques we investigated the interactions of superparamagnetic iron oxide nanoparticles (SPIONs) including maghemite γ-Fe₂O₃ and magnetite Fe₃O₄ with different coatings (e.g. citrate, chitosan) with transferrin. SPIONs present many advantages related to their magnetic properties, including magnetic manipulation and separation. The tools used allowed the characterization of the functionalized NP surface and the identification of structural changes of the proteins (e.g. circular dichroism spectroscopy) upon interaction with nanoparticles. In particular, dynamic light scattering measurements as well as SDS-PAGE revealed the corona formation of our model protein transferrin on the NPs, while transferrin also acted as a stabilizing agent of the colloidal suspension as verified by zeta potential measurements. In addition, the influence of the NPs on the iron binding site of transferrin in comparison to the binding to iron-free transferrin (apotransferrin) was studied via UV-Vis spectroscopy and urea PAGE.