Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 14: Cell mechanics I

BP 14.1: Vortrag

Mittwoch, 3. April 2019, 09:30–09:45, H10

A large scan area AFM for measurements on large biological samples and on many cells — •Todor Krastev, David Grunwald, and Tilman E. Schäffer — Universität Tübingen, Institut für Angewandte Physik, Auf der Morgenstelle 10, 72076 Tübingen

The atomic force microscope (AFM) has become a robust and versatile tool for the investigation of mechanical properties of biological samples. However, the scan range of typical AFMs (100 µ m in xy- and 10 µ m in z-direction) is limiting its use on large samples. We present a Macro-AFM with a scan range of 25 mm in xy- and 0.25 mm in z-direction. The Macro-AFM allowed us to map the elastic modulus of a cross section of a whole mouse aorta in a single scan. Furthermore, we applied image detection techniques to fluorescently stained cells to facilitate automated, autonomous measurements of many cells. We demonstrate the robustness of the method by comparing the elastic modulus of cells in a control group to a group treated with cytochalasin D. By performing measurements on over 1000 single cells per group, we achieved a remarkably low statistical p-value.