Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Cell mechanics I
BP 14.10: Vortrag
Mittwoch, 3. April 2019, 12:15–12:30, H10
High-throughput cell and tissue mechanics in virtual fluidic channels — •Muzaffar Hussain Panhwar1, Venkata A.S. Dabbiru1, Doreen Biedenweg2, Ricardo H. Pires1, Fabian Czerwinski1, and Oliver Otto1 — 1ZIK HIKE, University of Greifswald, Greifswald, Germany — 2University Medicine Greifswald, Greifswald, Germany
The biomechanical properties of cells and tissues are of utmost importance for a number of regulatory processes and complex functions, e.g., cell activation and migration. We use a novel development of real-time deformability cytometry (RT-DC) to access cell and spheroid mechanics of large samples. To accommodate the size range between cells and spheroids, we show that microfluidic geometries can be modified on-the-fly by polymer solutions creating virtual fluidic channels between two immiscible interfaces. After establishing virtual fluidic channels in the mesoscopic environment of a glass cuvette commonly used in flow cytometers, we explore their capability for high-throughput mechanical characterization. Performing RT-DC on an embryonic cell line cultured in 2D and comparing the results to several hundreds of spheroids as a 3D model system, a greater elastic modulus was found for single cells. These results might lead to a better understanding of tissue growth and could reveal insights into the mechanical dynamics of cell-cell interactions.