Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Cell mechanics I
BP 14.8: Vortrag
Mittwoch, 3. April 2019, 11:45–12:00, H10
Dynamic RT-DC: time-resolved mechanical single cell analysis at 100 cells / second — •Bob Fregin1, Fabian Czerwinski1, Konstanze Aurich2, Doreen Biedenweg2, Salvatore Girardo3, Stefan Groß4, and Oliver Otto1 — 1ZIK HIKE, Universität Greifswald, Greifswald, Germany — 2Universitätsklinikum Greifswald, Greifswald, Germany — 3Biotechnology Center, Technische Universität Dresden, Dresden, Germany — 4DZHK, Universität Greifswald, Greifswald, Germany
Real-Time Deformability Cytometry (RT-DC) is a label-free technique for single cell mechanical analysis with high-throughput of up to 1,000 cells / second. Capturing the steady-state deformation at the end of a microfluidic channel RT-DC is currently limited to time-independent parameters like the elastic Young's modulus.
Here, we introduce an extension of RT-DC towards dynamic single cell measurements with the possibility to capture full viscoelastic properties of up to 100 cells / second. Cellular shape-changes along the entire length of the microfluidic channel are tracked in real-time and are subsequently analyzed by a Fourier decomposition. We demonstrate that this dynamic RT-DC allows for cell mechanical assays at the millisecond time scale fully independent of cell shape. We use this approach for the first comparison of peripheral blood cells based on their elastic Young's modulus as well as their viscosity.