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Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 14: Cell mechanics I

BP 14.9: Vortrag

Mittwoch, 3. April 2019, 12:00–12:15, H10

The role of cell culture topology in cell mechanics: comparing 2D with 3D microenvironments — •Venkata AS Dabbiru1, Muzaffar H Panhwar1, Doreen Biedenweg2, Fabian Czerwinski1, Ricardo H Pires1, and Oliver Otto11ZIK HIKE, University of Greifswald, Greifswald, Germany — 2University Medicine Greifswald, Greifswald, Germany

Despite the widespread use of cell monolayers to culture cells in vitro, this 2D format does not recapitulate many of the physical cues present in the 3D environment that characterizes a tissue. In turn, these topological differences influence gene expression patterns and modulate the physiological behaviour of cells. However, the effect of topology on the mechanics of single cells has so far never been investigated systematically. Here, we apply real-time deformability cytometry (RT-DC) for the high-throughput mechanical phenotyping of single cells and cell spheroids. We compare HEK 293 cells obtained from planar monolayers (2D) and spheroidal (3D) formats and show that cells cultured in a 3D microenvironment have a larger Young's modulus when compared to those cultured in a 2D cell culture format. Furthermore, we show that cell confluency determines the average cell size but does not impact their mechanical properties.

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