Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Statistical physics of biological systems II (joint session BP/DY)
BP 28.2: Vortrag
Donnerstag, 4. April 2019, 15:15–15:30, H11
Unveiling lineage decisions in zebrafish neurogenesis — Emmanuel Than-Trong1,2, •Bahareh Kiani3, Alessandro Alunni1,2, Benjamin D. Simons4,5,6, Laure Bally-Cuif1,2, and Steffen Rulands3 — 1Institut Pasteur, Unit Zebrafish Neurogenetics, Department of Developmental & Stem Cell Biology, 25 rue du Dr Roux, 75015 Paris, France — 2CNRS, UMR3738, 25 rue du Dr Roux, 75015 Paris, France — 3Max-Planck-Institut für Physik komplexer Systeme, Nöthnitzer Straße 38, 01187 Dresden, Deutschland — 4Cavendish Laboratory, Department of Physics, University of Cambridge, Cambridge CB3 0HE, UK — 5The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK — 6The Wellcome Trust/Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge CB2 1QN, UK
Zebrafish neural tissue hosts specialised precursor cells which fuel the ongoing production of neurons into discrete brain regions. To understand how neural maintenance is achieved in this system, we performed a quantitative clonal analysis of the fate of precursor cells. Lineage tracing in growing tissues is complicated by the fact that labelled clones fragment into disconnected clusters, rendering the retrospective analysis of cell fate highly ambiguous. Combining statistical inference with biophysical modelling we reconstructed the clonal origin of labelled cells, revealing that progenitor containing clones persist over the lifetime of the animal. Using stochastic modelling, we unveiled lineage relationships and proliferation kinetics in the adult zebrafish pallium.