Regensburg 2019 – wissenschaftliches Programm
BP 28.3: Vortrag
Donnerstag, 4. April 2019, 15:30–15:45, H11
Revealing chromosome organization from Hi-C data using a maximum entropy approach — •Joris Messelink1, Jacqueline Janssen2, and Chase Broedersz1 — 1Arnold Sommerfeld Centre for Theoretical Physics, LMU Munich — 2Max Planck Institute for the Physics of Complex Systems, Dresden
The bacterial DNA outsizes the cell by roughly a factor of a thousand. The DNA must not only be highly condensed to fit inside the cell, but this condensed DNA must be organized inside the cell to facilitate functional processes of the chromosome. Thus, understanding the three-dimensional spatial organization of the bacterial chromosome is important to understand how the core biological processes are regulated inside of the cell. Recent chromosome conformation capture experiments provide genome-wide data on chromosome folding. In particular, the Hi-C method provides contact frequency maps of the chromosome, revealing its highly organized structure. We develop a maximum entropy approach to extract the three-dimensional structure of the bacterial chromosome from such data. The aim of our method is to develop a coarse-grained model for the statistical mechanics of the folding of the whole bacterial chromosome. From this model, we obtain the full distribution of chromosome configurations in the cell.