Regensburg 2019 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 3: Bioimaging and biospectroscopy I

BP 3.5: Vortrag

Montag, 1. April 2019, 10:30–10:45, H11

High throughput real-time measurements and image analysis of suspended cells and particles — •Daniel Geiger, Tobias Neckernuss, Jonas Pfeil, and Othmar Marti — Institute of Experimental Physics, University of Ulm, Germany

Imaging of cells has proven to be a viable tool to determine properties like type or pathogenicity. Recent advances in microfluidics and lab-on-a-chip devices are based on the availability of detection systems to observe single cells with very high throughput. High-speed cameras in such applications have several drawbacks. The large amount of data needs buffering, which in turn requires offline data evaluation. Furthermore, data evaluation by standard computer architectures introduces unpredictable latency between measurement and data analysis. Hence, applications such as sorting are hardly possible by a system built of conventional camera and data processing.

We present a novel device that is based on an advanced imaging system combined with a field programmable gate array (FPGA) for control and data analysis. Due to specially developed algorithms the FPGA is able to analyze the data in real time with a fixed latency. Therefore, applications based on image analysis that require a fixed and reliable latency are feasible. Our measurement system is able to analyze up to eight regions of interest, each running at 5000 frames per second, simultaneously.