Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 3: Bioimaging and biospectroscopy I
BP 3.8: Vortrag
Montag, 1. April 2019, 12:00–12:15, H11
Nanoscale dipole dynamics of protein membranes by Broadband Dielectric Microscopy — •G. Gramse1,3, A. Schönhals2, and F. Kienberger3 — 1JKU, Biophysics Institute, Linz, Austria — 2BAM, Berlin, Germany — 3Keysight Laboratories, Linz, Austria
The response of biological matter to electric fields is an intrinsic property in Biophysics which can be used to identify and characterize complex biological structures and sub-structures. At the same time, many physiological processes down to the cellular and sub-cellular level are based on electric and electrostatic interactions. Therefore quantitative investigation of dielectric properties at the nanoscale has gained major interest in recent years [1,2]. While dipoles at decreasing spatial scales within the biological structures relax with increasing frequencies, until now researchers could not address the frequency dependency of the dielectric permittivity and lacked a fundamental dimension for the understanding of many physical processes. We combined instrumentation for high frequency electrical characterization with the SPM and precise FEM based quantification procedures. The technique can be used to locally characterize biological micro-and nanoscale objects and allows for the first time quantitative nanoscale dielectric spectroscopy of bio-membranes in a broad frequency window from 3 kHz -10 GHz covering almost six orders of magnitude [3]. This allowed us to investigate the effect of surface water on the dipole dynamics in bR-membrane patches. The technique can be operated in dry and liquid environment. [1] Gramse G et al. 2013 Biophysical Journal 104 (p1257-62) [2] Biagi MC et al. 2016 ACS Nano 10, 1 [3] G. Gramse et al. Under Review