Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.36: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
Correlation of force generation and actin structure in blood platelets — •Anna Zelena1, Dimitri Probst2, Johannes Blumberg2, Ulrich S. Schwarz2, and Sarah Köster1 — 1Institute for X-ray Physics, Georg-August-University Göttingen, Göttingen, Germany — 2Institute for Theoretical Physics, Heidelberg University, Heidelberg, Germany
Human blood platelets are non-nucleated fragments of larger cells (megacaryocytes), which are important for blood clotting. The hemostatic function of platelets is directly linked to their mechanics and cytoskeletal morphology. However, the exact mechanism of spreading and contraction remains elusive. In our study, we focus on the investigation of single blood platelets in vitro by traction force microscopy (TFM) and direct imaging using an SiR actin probe. By analysis of bead displacements inside the polyacrylamid (PAA) gels, which serve as substrates for the platelets, we are able to correlate the force generation with the actin reoganization in a time resolved manner. The force maps we obtain show a hot spot distribution, typically in spindle-like, triangular or circular shape, that we correlate with actin structures inside spreading blood platelets. In addition, we investigate the actin structures of platelets on PAA gels with different elasticity in the physiological range (1-100 kPa) and their behavior in presence of micropatterned fibrinogen surfaces.