Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.48: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
The effect of reactive oxygen species on the molecular and mechanical phenotype of C6 glioma cells — •Yesaswini Komaragiri1, Doreen Biedenweg2, Ricardo H. Pires1, and Oliver Otto1 — 1ZIK-HIKE, Universität Greifswald, Greifswald, Germany — 2Universitätsmedizin Greifswald, Greifswald, Germany
Reactive oxygen species (ROS) are one of the main sources of oxidative stress which are associated with important alterations in cell physiology. Mechanical properties have long been established as a label-free biomarker but their interplay with alternating levels of ROS has not been fully investigated. This study focusses on understanding the impact of oxidative stress on the mechanical properties of immortalized rat brain C6 glioma cells. In an in vitro assay, mitochondrial superoxide was generated by exposing cells to varying concentrations of hydrogen peroxide. Using real-time fluorescence deformability cytometry, we link for the first time the molecular phenotype of ROS using MitoSOX-red as a fluorescent marker to changes in the mechanical phenotype as a label-free biomarker. We show that for micro-molar concentrations of H2O2, the elastic Young's modulus of the cells increases, which is in contrast to previous studies focusing on concentrations in the millimolar range.