Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.77: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
Sequence selection of oligonucleotides under a ligation chain reaction — •Patrick Kudella1 and Dieter Braun2 — 1Patrick.Kudella@physik.uni-muenchen.de — 2Dieter.Braun@lmu.de
The replication of information on RNA or DNA is central for the emergence of life (Szostak, 1990). Previously, the replication of one sequence has often been in the focus, but we think it is essential to monitor the replication and selection dynamics out of a completly random pool of sequences.
We focus on the transition from template-free polymerization to templated ligation. Once polymerization could create oligomers long enough to hybridize, we expect a nonlinear ligation dynamic to set in. We study whether sequences were selected at this onset of replication and if interesting non-linear and frequency-dependent behavior can be found (Toyabe, 2018).
We find, that for short strands, the ligation is dominated by the weak hybridization dynamics (ssDNA linked by Watson-Crick-base-pairing (Crick, 1970)). By using adenine-thymine-only 12mer random sequences as starting material, the sequence space for the first ligation stage that creates 24mer can still be completely sampled. We could obtain more than 12 million individual strands using Next Generation Sequencing (NGS), showing a significant selection of sequences undergoing this elongation dynamics. We analyze the sequences with self-written LabView code and show how spiking with defined sequences changes the sequence selection dynamics of the replicated and remaining sequence pool.