Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Bioimaging and biospectroscopy II
BP 7.8: Vortrag
Dienstag, 2. April 2019, 11:45–12:00, H4
Nanoscale X-ray computed tomography for the 3D structural characterization of electrospun fibers — Adriana T González1, Cristine S de Oliveira1, Tobias Kürbitz2,3, Christian E H Schmelzer3, Ralf B Wehrspohn1,3, and •Juliana Martins de S e Silva1,3 — 1Institute of Physics, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany — 2Anhalt University of Applied Science, Köthen, Germany — 3Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Halle (Saale), Germany
Electrospinning is a well-established method for the production of fibrous non-woven biomaterials which are used in tissue engineering as biomimetic three-dimensional (3D) scaffolds to assist cell growth. The properties of these non-woven biomaterials, including their porous network, the size, and interconnectivity of the electrospun fibers are critical parameters for their successful application as scaffolds. As the conventional methods for characterizing these fibers have deficiencies, such as the use of toxic substances and incomplete visualization of the porous structure, here we explored phase-contrast nanoscale X-ray computed tomography (nano-CT) as an alternative method to image and characterize the 3D structure of electrospun gelatin-based fiber matrices. The 3D datasets obtained gave a visual insight into the morphology of the fibers. We observed changes in fiber thickness upon chemical cross-linking, resulting in declines of porosity of 23 % and of the surface area of 39 %. We show that phase-contrast nano-CT is a promising method for the fast, non-destructive and high-resolution 3D imaging and quantitative characterization of electrospun nanofibers.