Regensburg 2019 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 17: Poster Session I
CPP 17.5: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B1
Water-Mediated Protein-Protein Interactions at High Pressures are Controlled by a Deep-Sea Osmolyte — Karin Julius1, Jonathan Weine1, Nico König1, Mimi Gao2, Jan Latarius1, •Michael Paulus1, Martin A. Schroer3, Metin Tolan1, and Roland Winter2 — 1Faculty of Physics/DELTA, TU Dortmund University, 44221 Dortmund, Germany — 2Faculty of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn-Strasse 4a, 44227 Dortmund, Germany — 3European Molecular Biology Laboratory (EMBL) Hamburg c/o DESY, Notkestrasse 85, 22607 Hamburg, Germany
Living cells accumulate organic osmolytes to high concentrations as adaptive response towards external stressors such as dehydration, freezing, salinity or high hydrostatic pressure as encountered in the deep sea. The mechanisms of protein stabilization and the linkage to the intermolecular interactions between proteins in the cellular milieu at ambient as well as at elevated pressures are still largely terra incognita. Herein, we study the impact of natural osmolyte mixtures on the pressure dependent intermolecular pair-interaction potential of dense protein solutions by applying small-angle X-ray scattering in combination with a liquid-state theoretical approach. Particularly, the presence of the deep-sea osmolyte TMAO guarantees the sustainability of the native protein fold under harsh environmental conditions. Further, we find a strong interplay between water and osmolyte in controlling the water-mediated intermolecular interactions at high pressure, thereby preventing contact formation and hence aggregation of proteins.