Regensburg 2019 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 17: Poster Session I
CPP 17.8: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B1
Secondary structure analysis of xanthan using atomic force microscopy — •Jenny Fjodorova1, Julia Voß2, Gerd Hublik3, Vera Ortseifen2, Karsten Niehaus2, Volker Walhorn1, and Dario Anselmetti1 — 1Experimental Biophysics and Applied Nanoscience, Bielefeld University, Germany — 2Proteome and Metabolome Research, Bielefeld University, Germany — 3Jungbunzlauer Austria AG, Pernhofen 1, 2064 Wulzeshofen, Austria
Xanthan is an extracellular polysaccharide, secreted by the bacterium Xanthomonas campestris. Due to its unique viscosifying properties over a wide range of salt concentrations, xanthan has numerous industrial applications e.g. in food, cosmetic or oil industry. Therefore, the optimisation of xanthan production and its rheological properties is of particular interest. Targeted genetic modification of the Xanthomonas metabolism and subsequent change of salt concentrations can be a powerful tool to optimise the shear-thickening potency and to improve the xanthan production efficiency.
Using atomic force microscopy (AFM) imaging and single molecule force spectroscopy (SMFS), we analysed the structure and the elastic characteristics of single xanthan polymers, which were produced by different Xanthomonas strains. We identified structures ranging from single-stranded coiled networks to branched double-strands. Taking into account the varying ability to form double-strands and differences in the bending stiffness, we observed a correlation between the formation of secondary structures and its resulting different viscosifying properties.