Regensburg 2019 – wissenschaftliches Programm

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MA: Fachverband Magnetismus

MA 51: Magnetism Poster B

MA 51.15: Poster

Donnerstag, 4. April 2019, 15:00–18:00, Poster C

Optimizing the cultivation of Magnetospirillum gryphiswaldense bacteria by setup of a hypoxic chamber — •Fabian Kugel, Paula Weber, Maryam Youhannayee, and Mathias Getzlaff — Institut für Angewandte Physik, Heinrich-Heine-Universität Düsseldorf, Deutschland

Nano-magnetic hyperthermia as a novel cancer therapy commonly uses iron oxide nanoparticles (NP) to heat up tumor tissue. Magnetotactic bacteria such as Magnetospirillum gryphiswaldense (MSR-1) synthesize magnetite NP by biomineralisation. These bacterial particles have unique properties that are advantageous in hyperthermia applications.

We established a cost-efficient method to cultivate MSR-1. The necessary microaerobic environment was implemented by setup of a hypoxic chamber and an oxygen control system.

The cultured bacteria were studied by transmission electron microscopy (TEM). According to the TEM images the quality of the cultivated samples was comparable to commercial products. The diameter of the internal NP was found to be (46.4±8.8) nm which corresponds to a magnetic single-domain state.

Further variations in the cultivation process where examined for their influence on the NP growth. The cultivation of MSN-1 at higher oxygen concentrations led to suppression of size in adolescent NP.