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BP: Fachverband Biologische Physik
BP 20: Poster VIII
BP 20.16: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/4OG
Local membrane height dynamics of live cells — •Max Ulbrich1, Christian Völkner1, Regina Lange1, Heiko Lemcke2, Robert David2, Martina Grüning3, Barbara Nebe3, Ingo Barke1, and Sylvia Speller1 — 1Institute of Physics, Physics of Surfaces & Interfaces, University of Rostock, 18059 Rostock — 2University Medical Center, Cardial Regeneration, University of Rostock, 18057 Rostock — 3University Medical Center, Dept. of Cell Biology, University of Rostock, 18057 Rostock
Cellular membrane fluctuations are considered for monitoring physiologic and pharmacologic effects [1]. Scanning Ion Conductance Microscopy (SICM) is a nanoprobing method to acquire morphologies on live cells. We operate the nanopipette-probe on fixed lateral locations and record SICM time traces in order to assess membrane fluctuations and cell activities with regard to processes [2]. Height variations of live osteoblasts and cardiomyocytes are analyzed in time and frequency domain. Cardiomyocytes show a pronounced frequency response behavior due to the electromechanical action, which can get modulated upon change of location or environment. Osteoblasts show rather strict 1/f behavior, with varying amplitude among different cells or cell surface regions. Close inspection points towards low frequency modulations in the course of migration. We discuss possible correlations with cell activity obtained by supporting optical microscopy.
[1] B Rappaz, et al, Blood Cells Mol. Dis. 42 (2009) 228
[2] S-O Kim, et al, Nano Convergence (2017) 4:5