Dresden 2020 – wissenschaftliches Programm
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BP 26.7: Vortrag
Mittwoch, 18. März 2020, 17:00–17:15, SCH A251
Simulation of cell deformation inside a microfluidic channel under the influence of a non-Newtonian fluid. — •Ralf Schuster1, Bob Fregin2, Oliver Otto2, and Othmar Marti1 — 1Institute of Experimental Physics, Ulm University, D-89081 Ulm — 2Humorale Immunreaktionen bei kardiovaskulären Erkrankungen, Universität Greifswald, D-17489
The mechanical characterization of certain cell types is important to obtain physiological insights. For instance, tumor and normal cells can be distinguished by elasticity, indicated by the amount of deformation under given stress. Simulations help to understand, verify and improve the analysis of deformation-based cell characterization such as flow-based cytometry. We achieve efficient computations using a 2D-rotational symmetric model, based on Fluid-Structure-Interaction with a hyper-elastic material. The deformation of a cell along an entire microfluidic channel can be tracked for a variety of elasticities, viscosities, cell sizes, channel geometries and flow rates. The aim is to model typical experimental conditions  and compare simulated and measured results. In the study HL60 cells undergo a shear stress in a fluid with shear dependent viscosity, which can be described by a power law. Simulations are carried out for three different flow rates and additionally for Newtonian fluids with constant viscosity. The results help to find appropriate parameters and models to describe and interpret the behavior of certain cell types.
Fregin et al, High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry. Nat Com(2019)