Dresden 2020 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 35: Bioimaging and Biospectroscopy II

BP 35.5: Vortrag

Donnerstag, 19. März 2020, 16:30–16:45, HÜL 386

Phase-Contrast X-Ray Tomography of Marmoset Cochlea — •Jannis Justus Schaeper1, Marius Reichardt1, Marina Eckermann1, Jasper Frohn1, Christoph Kampshoff2, Tobias Moser3, and Tim Salditt11Institute for X-Ray Physics, Göttingen University — 2Max-Planck-Institute for Experimental Medicine, Göttingen — 3InnerEarLab, University Medical Center, Göttingen

The cochlea is the receptor organ in the inner ear that transduces sound into neuronal activity. Both fundamental aspects of signal transduction and neuro-physiology as well as biomedical research (implant technology, hearing loss and disorders) requires three-dimensional (3D) imaging techniques capable to quantify the micro-anatomy.

We present optimized 3D imaging of excised small-animal cochleae by phase-contrast x-ray tomography using highly brilliant synchrotron radiation, and show how this technique can complement classical histology and light sheet microscopy in a correlative imaging approach. Shape, volumes and densities of individual neurons can be assessed.

In view of age-related hearing loss we particularly aim at quantitatively evaluating the number of spiral ganglion neurones and hair cells in different age groups of marmoset models. We show how high contrast for soft tissue [1] can be achieved, in particular using our endstation GINIX at DESY [2]. Due to high contrast and little noise, automated segmentation becomes possible. The CT-images are compared to lightsheet microscopy data to infer structural changes induced by the clearing process. [1] M. Töpperwien et al., Sci. Rep. 8, 4922 (2018), [2] T. Salditt et al., J Synchrotron Radiat. 22 (2015), 867-878

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