Dresden 2020 – wissenschaftliches Programm
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BP 35.7: Vortrag
Donnerstag, 19. März 2020, 17:00–17:15, HÜL 386
Dissection of Plasmodium falciparum developmental stages with multiple imaging methods — •Katharina Preißinger1,2, Beáta Vértessy1,2, István Készmárki3,4, and Miklós Kellermayer5 — 1Department of Applied Biotechnology and Food Sciences, BME, Budapest, Hungary — 2Institute of Enzymology, Research Center for Natural Sciences, Budapest, Hungary — 3Department of Physics, BME, Budapest, Hungary — 4Department of Experimental Physics V, University of Augsburg, Germany — 5Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
Every year, more than 200 million people are infected with malaria. The protozoon is transmitted into the human body by a mosquito bite. In the blood stream, malaria parasites invade red blood cells (RBC), mature to rings and trophozoites, multiply to schizonts and then burst out of the cells, ready to invade further ones. The digestion of haemoglobin by all Plasmodium species results in the accumulation of a metabolic byproduct and in morphological changes of the RBC, alterating topology and mechanics, which are typically characterized with bright-field microscopy (BF).
To explore correlations of the Plasmodium-induced molecular, topographical and mechanical changes, we investigated infected RBC with atomic force microscopy (AFM), phase contrast and total internal reflection fluorescence (TIRF) microscopy. By combining these imaging methods, we could correlate the morphological changes of RBC with the Plasmodium falciparum developmental stages.