Dresden 2020 – scientific programme

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BP: Fachverband Biologische Physik

BP 5: Bioimaging and Biospectroscopy I

BP 5.7: Talk

Monday, March 16, 2020, 17:00–17:15, HÜL 386

Particle tracking via an electrically focus tunable lens (ETL) — •Oliver Köhn and Christian Wagner — Universität des Saarlandes

We describe a method for the 3D tracking of particles using an electrically focus tunable lens (ETL). Applying a current to the ETL adapts the focal plane of the lens, allowing us to track particles in three dimensions without any mechanical interactions with the sample. Based on the sharpness of the tracked particles we are able to recalculate the current z-position of the particle. This calculation can be performed during the tracking-process, enabling us to perform live-tracking of particles. We show how this tracking-method can be applied to track 1.) bacteria (Bacillus subtilis) and 2.) um-sized beads, both for several minutes.