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DY: Fachverband Dynamik und Statistische Physik

DY 55: Poster: Turbulence; Complex Fluids; Microfluidics; Droplets and Wetting

DY 55.7: Poster

Thursday, March 19, 2020, 15:00–18:00, P1A

Upscaling of dielectrophoretic continuous-flow separation in a microfluidic system — •Jakob Derksen, Dario Anselmetti, and Martina Viefhues — Experimental Biophysics and Applied Nanoscience, Bielefeld University, Germany

High throughput analysis and separation of biomolecules or nanoparticles is an important task for many biological and medical applications such as separating DNA fragments and purification of gene vaccines. Thus, new separation techniques are requested. In this work, we present a sufficient microfluidic concept for upscaling of continuous-flow separation by means of dielectrophoresis (DEP).

DEP describes the movement of an electrically polarizable object in a non-uniform electric field. The advantages are that it is non-invasive, label-free and it provides selective manipulation of samples. A current disadvantage of this method is the limitation to small sample volumes and associated with that, the enormous purification time for reasonably sample volumes in the range of millilitre.

Here, we demonstrate the upscaling of a dielectrophoretic continuous-flow separation method in a microfluidic system. For that purpose, we created a system with two parallel separation channels in one device. We successfully demonstrated the separation of 100 nm and 40 nm beads and 10 kbp and 5 kbp DNA and harvesting in separate reservoirs. With these experiments, we firstly demonstrated an upscaling of separation throughput in a microfluidic device, paving the way to high-throughput applications.