Hannover 2020 – wissenschaftliches Programm
Die DPG-Frühjahrstagung in Hannover musste abgesagt werden! Lesen Sie mehr ...
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
Q: Fachverband Quantenoptik und Photonik
Q 20: Ultrashort Laser Pulses and Biophotonics
Q 20.7: Vortrag
Dienstag, 10. März 2020, 15:30–15:45, f435
Rapidly tunable all-fiber light source for live multicolor coherent Raman imaging — •Maximilian Brinkmann1,2, Tim Hellwig1,2, and Carsten Fallnich1 — 1Institute of Applied Physics, University of Münster, Germany — 2Refined Laser Systems GmbH, Münster, Germany
We present multicolor coherent Raman imaging (CRI) with rapid wavelength tuning within only 5 ms between successive images. In order to visualize rapidly evolving or moving samples in coherent Raman imaging (CRI) with high chemical specificity, successive images at multiple vibrational resonances have to be acquired at video-rate speed. Recent approaches to video-rate multicolor CRI, based on parallel laser amplifiers or spectral focusing techniques, allowed wavelength switching on a timescale of (sub)milliseconds, but only across a bandwidth of 300 cm−1 at maximum, significantly limiting the chemical specificity. In contrast, the here presented light source is tunable within 5 ms across the wide spectral range between 700 and 3530 cm−1. Therewith, the wavelength can be tuned in a frame-by-frame manner adequate for multicolor image acquisition with up to 100 frames/s. For a first demonstration, we have applied the light source for visualizing lipids and deuterated dimethyl sulfoxide (dDMSO) in mouse ear tissue with coherent anti-Stokes Raman scattering. Rapid switching of the excitation wavelengths to target 2130 cm−1 and 2845 cm−1 within only 5 ms allowed, without the need for a relative delay adjustment between pump and Stokes beam, to visualize how dDMSO has penetrated from the surface down to about 60 µm deep in the skin.