SKM 2021 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 2: Cytoskeleton
BP 2.4: Vortrag
Montag, 27. September 2021, 12:30–12:45, H1
Bottom-up assembly of functional DNA-based cytoskeletons for synthetic cells — •Kevin Jahnke1,2, Pengfei Zhan3,4, Na Liu3,4, and Kerstin Göpfrich1,2 — 1Max Planck Institute for Medical Research, Heidelberg, Germany — 2Heidelberg University, Heidelberg, Germany — 3Stuttgart University, Stuttgart, Germany — 4Max Planck Institute for Solid State Research, Stuttgart, Germany
Bottom-up synthetic biology aims at reconstructing a cell from biomolecular constituents. However, the combination of multiple elements and functions remained elusive, which stimulates endeavors to explore entirely synthetic bio-inspired solutions towards engineering life. To this end, DNA nanotechnology represents one of the most promising routes, given the inherent sequence specificity, addressability, and programmability of DNA. Here, we demonstrate functional DNA-based cytoskeletons operating in microfluidic cell-sized compartments and lipid vesicles. The synthetic cytoskeletons consist of DNA tiles self-assembled into filament networks. These filaments can be rationally designed and controlled to imitate features of natural cytoskeletons, including dynamic instability, ATP-triggered polymerization, and vesicle transport in cell-sized confinement. Also, they possess engineerable characteristics, including assembly and disassembly powered by DNA hybridization or aptamer-target interactions and autonomous transport of gold nanoparticles. This work underpins DNA nanotechnology as a key player in building synthetic cells.