SKM 2021 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur
KFM 5: Poster Session KFM
KFM 5.9: Poster
Dienstag, 28. September 2021, 16:00–17:00, P
In-vivo tracking of potassium niobate nanoparticles by means of the TIGER microscope — •Laura Vittadello1, Jan Klenen1, Karsten Koempe2, and Mirco Imlau1 — 1Department of Physics, Osnabrueck University, Germany — 2Department of Biology/Chemistry, Osnabrueck University
In recent year, remarkable progress in the area of in-vivo harmonic nanoparticle (HNPs)-based nonlinear optical (NLO) microscopy has been reported. From one side the NLO microscopy has emerged as a successful tool within the bio-medical research field enabling the imaging of intact living organisms. From the other side, polar ferroelectric HNPs have been identified as a good marker candidate in such type of technique for their high nonlinear optical coefficients. Despite of this success, realtime in-vivo tracking based on HNPs has not been exploited so far, mainly because of a lack of an appropriate microscopy tool, i.e. a nonlinear optical widefield microscope. We realised this by means of a regeneratively amplified fs-laser coupled to an inverted microscope creating an easy alignable and reproducible Tunable hIGh Energy (TIGER) widefield microscope [Vittadello et al. Opt. Mater. Express 11, 1953-1969 (2021)]. This new approach is successfully applied for HNPs tracking in a area up to 1.5 x 1.5 mm2 in the blood flow of the heart system of a Drosophila larvae, a powerful platform to study social relevant diseases, such as congenital heart defects in human beings. The goal is to access the blood circulation in the heart of a larve, a quantity directly linked to the presence of cardiac disease. Financial support (DFG INST 190/165-1) is gratefully acknowledged.