SAMOP 2023 – wissenschaftliches Programm
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Q: Fachverband Quantenoptik und Photonik
Q 22: Poster II
Q 22.86: Poster
Dienstag, 7. März 2023, 16:30–19:00, Empore Lichthof
Structuring hydrogels by two-photon lithography inside microfluidic channels for cell migration experiments — •Elena Bekker1, Dustin Dzikonski1, Riccardo Zamboni1, Jörg Imbrock1, and Cornelia Denz1,2 — 1Insitute of Applied Physics, University of Münster, Corrensstraße 2-4, 48149 Münster, Germany — 2Physikalisch-Technische Bundesanstalt, Budesallee 100, 38116 Braunschweig, Germany
Hydrogels are highly swellable polymers which generally possess excellent biocompatibility and tissue-like properties. They can be structured via direct laser writing by two-photon lithography (2PL), enabling the fabrication of three-dimensional arbitrary structures with high resolution and spatial complexity. Using this technique, the microenvironment of cells can be mimicked with a high degree of control and reproducibility, whilst allowing variation in the physical properties of the structured gels. We perform 2PL fabrication inside channel systems of microfluidic devices, which provide a three-dimensional culture chamber for cells and allow the generation of chemical gradients to stimulate directed migration towards chemoattractant species. In this way, we investigate cell migration in confined three-dimensional environments.