SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster Session I
BP 11.10: Poster
Dienstag, 28. März 2023, 12:30–15:30, P1
FTIR and SRE spectra analysis for supported lipids bilayers (SLB's) with dry incorporation of Gramicidin A — •D. Saavedra1, N. Moraga1, N. Gomez-Vierling1, M. Cisternas2, R. Rodriguez1, S. Rojas2, and U.G. Volkmann1 — 1Institute of Physics and CIEN-UC, Pontificia Universidad Catolica de Chile — 2School of Industrial Engineering, Universidad de Valparaiso, Santiago, Chile
A dry method for SLB's assembling was developed in our group, without use of solvents and in vacuum [1], with the aim of synthesizing stable platforms for biosensors. For characterization, FTIR spectrum was analyzed for the detection of functional groups of DPPC and DSPC phospholipids in the range of 800 - 4000 1/cm. Using the SRE spectrum of DPPC and DSPC, their phase transitions were studied as a function of temperature. The SLB's/Gramicidin interaction at different concentrations were analyzed in order to optimize the growth of the biomolecules. These results would allow to evaluate the use of spin-probes in Gramicidin for the study of ion channel formation [2] and as a prototype for insertion of larger proteins. Acknowledgments: Fondecyt 1180939 (UGV), ANID doctoral grants (NM and NGV) and ANID SIA SA77210032 (MC and SR).
References [1] M. A. Cisternas, et al., Int. J. Mol. Sci. 21 (18), (2020) 6819. [2] Dzikovski, B.G., et al., J. Phys. Chem. B 2011, 115(1), 176-185.