SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster Session I
BP 11.11: Poster
Dienstag, 28. März 2023, 12:30–15:30, P1
Detection of Gramicidin by DPH fluorescence technique in supported phospholipids bilayers (SLB's) on SiO2 substrate — •D. Saavedra1, M. Soto-Arriaza2, N. Moraga1, N. Gomez-Vierling1, M. Cisternas3, and U.G. Volkmann1 — 1Institute of Physics and CIEN-UC, Pontificia Universidad Catolica de Chile — 2Faculty of Medicine and Science, Universidad San Sebastian, Santiago, Chile — 3School of Industrial Engineering, Universidad de Valparaiso, Santiago, Chile
An unconventional method to manufacture supported lipid bilayers (SLB's) was developed in our laboratory: without solvents, dry [1,2] and in the absence of gases, with the aim of synthesizing stable biosensor platforms. In this work we use our physical fabrication method for the incorporation of specific signal transmitters that have selective sensitivity.
The fluorescence emission spectra of Gramicidin, with DPH as extrinsic probe and fluorescence resonant energy transfer (FRET) techniques, seeks to detect its incorporation into the SLB's.
A series of samples were prepared in absence and presence of Gramicidin and the extrinsic probe DPH. Detection was realized using a single time-correlated spectrofluorimeters photon counting (TCSPC).
Acknowledgments: Fondecyt 1180939 (UGV), ANID doctoral grants (NM and NGV).
References: [1] Cisternas Fruns, M. A. (2021). Ph.D. Thesis, PUC, Chile. https://repositorio.uc.cl/handle/11534/60584. [2] M. A. Cisternas, et al., Int. J. Mol. Sci. 21 (18), (2020) 6819.