SKM 2023 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 11: Poster Session I

BP 11.35: Poster

Dienstag, 28. März 2023, 12:30–15:30, P1

phospholipids diffusion on the surface of model lipid droplets — •Shima Asfia, Ralf Seemann, and Jean-Baptiste Fleury — Universitüt des Saarlandes, Experimental Physics and Center for Biophysics, 66123 Saarbrücken, Germany

Lipid droplets (LD) are organelles localized in the membrane of the Endoplasmic Reticulum (ER) that play an important role in metabolic functions. Many studies have focused on the biophysical properties of these LDs. However, despite numerous efforts, we are lacking information on the mobility of phospholipids on the LDs surface, although they may play a key role in the protein distribution. In this article, we developed a microfluidic setup that allows the formation of a triolein*buffer interface decorated with a phospholipid monolayer. Using this setup, we measured the motility of phospholipid molecules by performing Fluorescent Recovery After Photobleaching (FRAP) experiments for different lipidic compositions. The results of the FRAP measurements reveal that the motility of phospholipids is controlled by the monolayer packing decorating the interface [1].

[1]S. Asfia, R. Seemann and J-B. Fleury, BBA-Biomembranes, 1865, 1 (2023).