SKM 2023 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 11: Poster Session I
BP 11.41: Poster
Dienstag, 28. März 2023, 12:30–15:30, P1
A preparative mass spectrometer to deposit intact large native protein complexes — •Paul Fremdling1, Tim K. Esser1, Bodhisattwa Saha1, Alexander A. Makarov2,3, Kyle L. Fort2, Maria Reihnhardt-Szyba2, Joseph Gault1, and Stephan Rauschenbach1,4 — 1Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK — 2Thermo Fisher Scientific, Bremen, 28199, D — 3Bijvoet Center for Biomolecular Research, University of Utrecht, Padualaan 8, 3584 CH Utrecht, NL — 4MPI for Solid State Research, Heisenbergstrasse 1, Stuttgart, 70569, D
Electrospray ion-beam deposition (ES-IBD) is a tool to study structure and reactivity of nonvolatile molecules. It ionises molecules gently, purifies and deposits them onto a substrate. In combination with imaging techniques, direct structural information can be obtained.
There are only a small number of custom ES-IBD instruments worldwide, with no commercial ones. We present a module that adds ion-beam deposition capabilities to a commercial MS (Thermo ScientificTM Q ExactiveTM UHMR).
We characterise beam intensity, landing-energy control, and deposition spot size for a broad range of molecules. In combination with atomic force microscopy (AFM) and transmission electron microscopy (TEM), we distinguish near-native from unfolded proteins and show retention of native shape of protein assemblies after dehydration and deposition. Further, we use an enzymatic assay to quantify activity of a non-covalent protein complex after deposition on a dry surface.