SKM 2023 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 11: Poster Session I

BP 11.54: Poster

Dienstag, 28. März 2023, 12:30–15:30, P1

Band formation of red blood cells by density gradient centrifugation — •Luca David Hastenteufel, Felix Milan Maurer, and Christian Wagner — Experimentalphysik, Universität des Saarlandes, Saarbrücken

Percoll is a commercial density medium consisting of coated silicia particles, which show a non-toxiticy to cells and low surface charge. Nowadays, Percoll is the standard medium for density separation of erytrocytes, leukocytes and other subcellular particles. The distribution of red blood cells after centrifugation in a self-forming Percoll gradient is characterized by a heterogeneous structure of discrete bands. We established a one dimensional particle model and a set of experiments to show that band formation is caused by aggregation. We also developed a continuum model describing the development of the RBC volumetric density under influence of a pair interaction. It shows also discrete solutions in the shape of band patterns. Understanding the band patterns gives information on the aggregation energy and disease severeness.