SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster Session I
BP 11.9: Poster
Dienstag, 28. März 2023, 12:30–15:30, P1
A NAP-XPS-study on X-ray radiation damage: Chemical changes to Gene-V Protein — •Dorothea C Hallier1,2,3, Jörg Radnik2, Paul M Dietrich4, Harald Seitz1,3, and Marc Benjamin Hahn2 — 1Fraunhofer Insitute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany — 2Federal Insitute for Materials Research and Testing BAM Berlin, Berlin, Germany — 3Univerity of Potsdam, Institute for Biochemistry and Biology, Potsdam Germany — 4SPECS Surface Nano Analysis GmbH, Berlin, Germany
Single-stranded DNA-binding proteins such as Gene-V Protein (G5P/GVP) are involved in maintaining the DNA metabolism after exposure to ionizing radiation, i.e. after radiation therapy in cancer treatment. X-ray photoelectron spectroscopy (XPS) was used to analyze the chemical damage of ionizing radiation to G5P itself. Direct and indirect damage was detected through combined vacuum XPS and near-ambient pressure (NAP) XPS measurements under water and nitrogen atmosphere. The x-ray irridation leads to degradation i.e. via dehydrogenation, decarboxylation, dehydration and deamination. A strong increase of protein damage was observed in water as compared to vacuum.