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BP: Fachverband Biologische Physik
BP 12: Biopolymers and Biomaterials (joint session BP/CPP)
BP 12.7: Talk
Wednesday, March 29, 2023, 11:30–11:45, TOE 317
3D stimulated Raman spectral imaging of water dynamics associated with pectin-glycocalyceal entanglement — •Moritz Floess1, Tobias Steinle1, Florian Werner1, Yunshan Wang1, Willi L. Wagner2, Verena Steinle2, Betty S. Liu3, Yifan Zheng3, Steven J. Mentzer3, and Harald Giessen1 — 14th Physics Institute, University of Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany — 2Department of Diagnostic and Interventional Radiology, University Hospital of Heidelberg, Im Neuenheimer Feld 420, 69120 Heidelberg, Germany — 3Laboratory of Adaptive and Regenerative Biology, Brigham & Women's Hospital, Harvard Medical School, Boston MA
Pectin, a heteropolysaccharide, is an ideal biomaterial for medical applications such as serosal wound healing and visceral tissue repair. It forms strong mechanical bonds with the underlying tissue. The extraordinary adhesive properties of pectin on organ surfaces are highly water-dependent and most likely result from a microstructural entanglement of pectin polysaccharide chains with the similarly textured glycocalyx, a glycoprotein coat, covering mammalian cell surfaces. We employ label-free 3D stimulated Raman scattering (SRS) microscopy to investigate the hydrophilicity of pectin hydrogel without the altering effects of sample fixation, dehydration, or tissue staining. In particular, we quantify the time scales, on which two different hydration mechanisms take place. Furthermore, the transition zone between pectin and porcine serosal tissue is imaged to obtain quantitative insights into the entanglement between pectin and mammalian glycocalyx.