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BP: Fachverband Biologische Physik
BP 17: Protein Structure and Dynamics
BP 17.1: Talk
Wednesday, March 29, 2023, 15:00–15:15, BAR 0106
Computational Approaches to Liquid-Liquid Phase Separation of Partially Disordered RS-Proteins — •Yannick Witzky1, Stephan Hobe2, Andreas Wachter2, Arash Nikoubashman1, and Friederike Schmid1 — 1Institute of Physics, Johannes Gutenberg University — 2Institute for Molecular Physiology, Johannes Gutenberg University
RS-proteins are a class of proteins that contribute to light-activated gene regulation (via an alternative splicing mechanism) in plant morphogenesis. It has been hypothesized that liquid-liquid phase separation (LLPS) plays an important role for the regulation mechanism. Studying these proteins is challenging because they contain both intrinsically disordered regions (IDRs) - which presumably control the LLPS - as well as folded domains that contain the functionally important RNA binding sites. Here we use and compare different coarse-grained models to study the condensation and phase behavior of RS proteins: Commonly used IDP models [1,2] as well as the structure predictive UNRES model [3]. We specifically focus on the on the single-chain conformations, phase behavior and the accessibility of RNA binding site.
[1] Tesei et al. (2022) Open Research Europe, 2(94), 94.
[2] Rizuan et al. (2022) J Chem Inf Model 62(18), 4474-4485.
[3] Sieradzan et al.(2019) J. Phys. Chem. B, 123, 27, 5721-572