SKM 2023 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 21: Bioimaging
BP 21.9: Vortrag
Donnerstag, 30. März 2023, 12:00–12:15, BAR Schö
Investigating human lung tissue by propagation-based phase-contrast X-ray tomography — •Jakob Reichmann1, Stijn Verleden2, Mark Kühnel3, Jan-Christopher Kamp3, Lavinia Neubert3, Jan-Hendrik Müller1, Thanh Quynh Bui1, Danny Jonigk3, and Tim Salditt1 — 1Institute for X-ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen — 2Anatomy and Research Centre, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium — 3Institute of Pathology, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover
The lung is a perfect example of how the function of an organ is enabled by its three-dimensional structure, here formed by intricate and intertwined networks of ventilation and vasculature. In this work we study the structure of lung tissues over multiple scales down to the sub-cellular level by phase-contrast computed tomography (PCCT). We show how human lung tissues with their largely air-filled compartments and small vessels can be imaged non-destructively with a scalable, isotropic resolution and quantitative density. Three-dimensional reconstructions with varied voxel sizes down to 130nm are obtained by advanced phase retrieval and tomographic reconstruction, shedding light on the three-dimensional cytoarchitecture. Morphometric parameters are extracted by automated image processing, and used to quantify the degree of pathological alterations. This offers unique potential to extend histology and pathohistology to study e.g. SARS-CoV-2 infected tissue or other lung degrading diseases such as COPD or cystic fibrosis, as we show with first applications of the method.