SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.15: Poster
Donnerstag, 30. März 2023, 18:00–20:00, P2/EG
A backward-mode optical-resolution photoacoustic microscope for functional 3D imaging — •Elisabeth Baumann1,2, Ulrike Pohle3, Thomas Allen4, Holger Gerhardt1,2, and Jan Laufer3 — 1MDC, Berlin, DE — 2Charite, Berlin, DE — 3MLU, Halle, DE — 4UCL, London, UK
Optical-resolution photoacoustic microscopy (OR-PAM) is a biomedical imaging technique with great potential for preclinical studies of the vasculature. It can be used to obtain spatially resolved information on parameters like the blood flow speed and blood oxygen saturation. However, for many in vivo applications, backward-mode operation of the OR-PAM system is required for which conventional piezoelectric ultrasound sensors are at a disadvantage as they need to be placed far away from the signal source. Here, we present an all-optical, backward-mode OR-PAM system that fulfils the requirements for functional imaging of the vasculature. The system incorporates a novel planar Fabry-Pérot (FP) ultrasound sensor, which is transparent to the excitation light and can be placed immediately adjacent to the sample.
To demonstrate the morphological imaging capabilities of the system, we performed 3D imaging in vitro of a leaf skeleton phantom and in vivo of a zebrafish embryo in raster-scanning mode. To be able to resolve blood flow, a fast continuous-scanning mode was established and validated in imaging phantoms. To show the capabilities of the system to image blood oxygen saturation, a Raman laser was set up for multi-wavelength excitation OR-PAM of ink phantoms.