SKM 2023 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.29: Poster
Donnerstag, 30. März 2023, 18:00–20:00, P2/EG
Exploring cell-cell interactions inferred from trajectories in two-site arrays — •Emily Brieger1, Tom Brandstätter2, Georg Ladurner1, Chase P. Broedersz2, and Joachim O. Rädler1 — 1LMU, Munich, Germany — 2VU Amsterdam, Amsterdam, Netherlands
Collective cell migration is a fundamental aspect to a variety of physiological processes. The migratory dynamics of these collective processes rely on cell-cell interactions that are depending on complex molecular mechanisms, such as cadherin dependent pathways. In previous work we studied the interaction behavior of two cells migrating on dumbbell-like micropattern. This geometry enforces repeated head-to-head collisions of cells and allows the distinction of interacting and noninteracting events. In this framework non-cancerous MCF10A cells and cancerous MDA-MB-231 cells clearly differ in their effective adhesion and friction terms derived from quantitative theoretical analysis. Here we use this data-driven approach to study how specific molecular alterations of surface proteins change cell-cell interactions in different cell lines. Blocking of E-cadherin and Ephrin A2 via antibodies yield distinct shifts in cell behavior space. Likewise knockouts that prevent cells from using their usual communication pathways alter the adhesive and frictional interactions. We show that the analysis yields insight into the role of E-Cadherin and Ephrin A2 in frictional and adhesive interactions as well as repulsive response known as contact inhibition of locomotion.