SKM 2023 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 28: Poster Session II
BP 28.30: Poster
Donnerstag, 30. März 2023, 18:00–20:00, P2/EG
A high-throughput pipeline for morphological and functional analysis of cardiomyocytes — Daniel Härtter1,2, •Lara Hauke1, Wolfram-Hubertus Zimmermann1, and Christoph F. Schmidt2 — 1Institute of Pharmacology and Toxicology, University University Medical Center Göttingen, Germany — 2Department of Physics, Duke University, Durham, NC, USA
Cardiomyopathies, diseases of the heart muscle, affect 1 in 500 adults in Western countries. Nevertheless, reliable knowledge about disease onset and pathogenesis is lacking. To develop effective treatment options for patients, a dynamic and quantitative understanding of cardiomyopathies is needed. We developed an assay in which individual stem-cell derived heart cells of a fluorescent sarcomere reporter cell line grow in a heart-like environment - while allowing for automated high-resolution and high-framerate imaging - using micropatterned polyacrylamide acid (PAA) gels. We analyze the time-course of cell morphology and function upon drug-induced or genetic interventions with our deep-learning-based SarcAsM (Sarcomere Analysis Multitool) software. The resulting multiparametric functional and structural trajectories of cardiac muscle cells can be used to gain novel dynamical perspectives on the time-course and interplay of structure and function in health and disease and might contribute to the discovery of novel treatments.