SKM 2023 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 28: Poster Session II

BP 28.8: Poster

Donnerstag, 30. März 2023, 18:00–20:00, P2/EG

Organelle organization and dynamics in cells on soft substrates — •Paula Girones Paya, Florian Rehfeldt, and Matthias Weiss — Experimental Physics I, University of Bayreuth, Germany

The interior of eukaryotic cells features a highly organized and dynamically evolving set of compartments (organelles), e.g. mitochondria, the endoplasmic reticulum, or the nucleus. How these organelles self-organize to arrive at an arrangement that is beneficial for a cell is still poorly understood. Numerous studies have monitored organelle morphologies and dynamics in well-characterized culture cells grown on rigid substrates, e.g. glass coverslips. In real life, however, cells are situated in soft tissues with an elastic modulus that is five to six orders of magnitude lower. On soft substrates, actin-based stress fibers are less pronounced, and consequently cell morphologies are considerably less flattened by tensile forces, suggesting that also organelle arrangement and dynamics is altered. Here, we compare results of mitochondrial dynamics, cell nucleus volumes, and the exchange kinetics for diffusively driven transport between the nucleoplasm and the cytoplasm of cells on soft and rigid substrates.