SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 31: Cell Mechanics III
BP 31.3: Vortrag
Freitag, 31. März 2023, 10:30–10:45, BAR 0106
Viscoelastic characterization of biological cells in hyperbolic microfluidic channels — •Felix Reichel1,2 and Jochen Guck1,2 — 1Max Planck Institute for the Science of Light, Erlangen, Germany — 2Max-Planck-Zentrum für Physik und Medizin, Erlangen, Germany
Research over the last decades revealed that single-cell mechanical properties can serve as label-free markers of cell state and function and that mechanical changes are a sign of alterations in the cell's molecular composition. This led to the development of a number of microfluidics tools to rapidly measure the deformability and also the viscoelastic properties of cells. The quantification of the stresses, that cause the deformation of the cells in these channels, is often challenging and with that the derivation of a stress-strain relation for such a system becomes complex. Here, we used hyperbolic channels to create an extensional flow field where the acting stresses can be measured using calibration particles and yield a simple relationship between acting stress and resulting cell strain. We then used the setup to measure the Young's modulus and bulk viscosity of HL60 cells and blood cells over a wide range of time scales. Drug induced changes to the cell state could be measured by a change in cell mechanical properties. Our simple setup offers a straightforward measurement of the viscoelastic properties of cells and microscale soft particles.