SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 31: Cell Mechanics III
BP 31.4: Vortrag
Freitag, 31. März 2023, 10:45–11:00, BAR 0106
Pancreatic cancer metastasis: mechanics and adhesion — •Shruti G. Kulkarni1, Malgorzata Lekka2, and Manfred Radmacher1 — 1Institute of Biophysics, University of Bremen, Otto-Hahn Allee 1, 28359 Bremen, Germany — 2Institute of Nuclear Physics PAN, Radzikowskiego 152 , 31-342 Krakow, Poland
We use Atomic Force Microscopy (AFM) to characterise the mechanical properties of Pancreatic ductal adenocarcinoma (PDAC) cell lines from the primary tumour site (PANC1), and from liver (CFPAC1) and lymph node (Hs766T) metastases. AFM measures forces using the optical lever system. To measure their stiffness, cells were probed using a rectangular cantilever with a three-sided pyramidal tip. Apparent Young's modulus (E) and power-law exponent (α) can be calculated from the force curves. To probe the adhesive properties of the cells, a single cell was attached to functionalised triangular tipless cantilevers, and then pressed against a confluent layer of cells. When the cell-cantilever is retracted, the contact of the attached cell with the cell layer is broken. The detachment exerts force on the cantilever, and this signal is also recorded by and characterised from the retract curve. The adhesion of cancer cells was measured with self-cells (the same cell line) as well as with endothelial cells (EA.hy926). CFPAC1 cells soften in confluent layers, and have increased cell-cell interaction with endothelial cells as compared to self-cells. Hs766t have similar stiffness as both single cells and as a confluent layer, and have higher cell-cell interaction with self-cells than with endothelial cells.