SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Cell Mechanics I
BP 8.8: Vortrag
Dienstag, 28. März 2023, 12:00–12:15, BAR Schö
Impact of oxidative stress on the mechanical properties of isolated mitochondria — •Yesaswini Komaragiri1,2, Muzaffar H Panhwar1,2, Bob Fregin1,2, Gayatri Jagirdar3, Carmen Wolke3, Stefanie Spiegler1,2, and Oliver Otto1,2 — 1Institut für Physik, Universität Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17489 Greifswald, Germany — 2DZHK, Greifswald, Universitätsmedizin Greifswald, Fleischmannstr. 42, 17489 Greifswald, Germany — 3Universitätsmedizin Greifswald, Ferdinand-Sauerbruch-Strasse, 17475 Greifswald, Germany
Mitochondria are essential in various physiological processes, including the homeostasis of reactive oxygen species (ROS) as key intracellular signaling molecules. While it is already established that mechanical properties are a crucial parameter in characterizing and comprehending biological systems such as cells and tissues, little is known about the significance of organelle mechanics for cell function. Here, we demonstrate the application of real-time fluorescence and deformability cytometry for the label-free and high-throughput analysis of mitochondria isolated from C6 glial cells. Our data on several thousands of viable mitochondria indicate that their deformation is shear stress dependent. We studied the effect of exogenously and endogenously generated ROS on mitochondria mechanics in two proof-of-concept studies. Under both conditions, we observed a decrease in size while the deformation increased relative to a control condition. The results suggest a general biophysical mechanism of how mitochondria respond to oxidative stress.