SKM 2023 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Cell Mechanics I
BP 8.9: Vortrag
Dienstag, 28. März 2023, 12:15–12:30, BAR Schö
New applications for the direct method in 3D traction force microscopy — •Johannes Blumberg1,2, Simon Brauburger1,2, and Ulrich Schwarz1,2 — 1Institute for Theoretical Physics, Heidelberg University — 2Bioquant, Heidelberg University
Traction force microscopy (TFM) estimates the mechanical forces of cells adhering to an elastic substrate by measuring by the movement of embedded marker beads. It has become a standard tool to study the mechanobiology of single cells or cell monolayers on flat two-dimensional (2D) substrates, but three-dimensional (3D) setups provide new challenges. Although the inverse method of force inference by minimization of a loss function has become the standard method for 2D TFM, for 3D TFM the direct method of directly calculating stress and surface traction from strain becomes an attractive alternative, for example when performing 3D TFM with elastic beads in organisms, tumor spheroids or organoids. We explain how this method works in practice and how it compares to the inverse method.