SKM 2023 – wissenschaftliches Programm
DY 4.2: Vortrag
Montag, 27. März 2023, 09:45–10:00, ZEU 250
Band Pattern Formation in a Suspension of Red Blood Cells During Centrifugation in a Percoll Density Gradient — •Felix Maurer, Thomas John, Christian Wagner, and Alexis Darras — Dynamics of Fluids, Experimental Physics, Saarland University, 66123 Saarbrücken, Germany
Percoll is a suspension of silica nanoparticles often used to establish density gradients and separate biological matter in centrifugation protocols. When red blood cells (RBCs) sediment in a Percoll medium, they form patterns of discrete bands. While this is a popular approach for RBC age separation, the mechanisms involved in band formation were unknown. In a series of experiments we could show that the formation of those patterns could be explained by cell aggregation. We developed a new continuum model to describe the volumetric RBC density under the influence of attractive pair interaction. Our numerical solutions are characterized by pattern formation and transitions between the equilibrium states depending on aggregation energy and initial volumetric RBC concentration.